Description& Advantages

Phosphorylation of proteins and lipids is a hallmark of the signaling or metabolic status of cells. A cocktail of three inhibitors that will inhibit alkaline phosphatases as well as serine/threonine protein phosphatases such as PP1 and PP2A. Each vial contains 1 ml phosphatase inhibitor cocktail solubilized in DMSO with the following components: Bromotetramisole oxalate (2.5 mM), Cantharidin (500 μM), and Microcystin-LR , Microcystis aeruginosa (500 nM).

Phosphatase Inhibitor Cocktail I has been optimized and tested for mammalian cell and tissue extracts. This cocktail is supplied as a ready-to-use solution in DMSO.

Publications

• ?Adv Funct Mater. 2020, 2004886.

• ?Signal Transduct Target Ther. 2022 Feb 25;7(1):53.

• ?Signal Transduct Target Ther. 2021 Sep 4;6(1):333.

• ?Signal Transduct Target Ther. 2021 Aug 30;6(1):322.

• ?Mol Cell. 2022 Apr 8;S1097-2765(22)00265-9.

• ?Hepatology. 2021 Jan;73(1):160-174.

• ?Neuron. 2022 Jan 20;S0896-6273(21)01090-4.

• ?Adv Sci (Weinh). 2021 Nov 17;e2103065.

• ?ACS Nano. 2020 Mar 24;14(3):3059-3074.

• ?Nat Commun. 2020 Aug 26;11(1):4254.

• ?EMBO Mol Med. 2022 Mar 7;14(3):e14455.

• ?EMBO Mol Med. 2020 Dec 7;e12798.

• ?Theranostics. 2021 Mar 4;11(10):4825-4838.

• ?Theranostics. 2021 Jan 1;11(2):925-940.

• ?Theranostics. 2020 Oct 30;10(26):12223-12240.

• ?Clin Transl Med. 2021 Mar;11(3):e359.

Price Comparison

Storage

The product should be stored at -20°C, and is stable for up to 12 months. When kept at 2-8°C, the product remains stable for 2 months.

Protocol

Thaw at room temperature. Before assaying, add each solution at 1:100 (v/v) dilution to samples (such as cell lysates or tissue extracts). Mix the sample sufficiently after each time of addition.

Application Fields: Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.

Download Inhibitor Cocktail Flyer 

https://www.medchemexpress.cn/Inhibitor/MCE_Inhibitor_Cocktails_cn.pdf