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免疫球蛋白G(IgG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
編號CEA544Gu
物種Cavia (Guinea pig ，豚鼠) 相同的名稱，不同的物種。
實驗方法競爭抑制
反應(yīng)時長2h
檢測范圍1.23-100ug/mL
靈敏度最小可檢測劑量小于等于0.48ug/mL.
樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
下載英文說明書 中文說明書
規(guī)格48T96T 96T*5 96T*10 96T*100
價格￥ 1840
產(chǎn)品包裝圖模擬
特異性
本試劑盒用于檢測免疫球蛋白G(IgG)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的免疫球蛋白G(IgG)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 86-101 | 95 |
| EDTA plasma(n=5) | 90-102 | 97 |
| heparin plasma(n=5) | 84-91 | 87 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的免疫球蛋白G(IgG)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中免疫球蛋白G(IgG)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-96% | 80-98% | 86-105% | 89-101% |
| EDTA plasma(n=5) | 86-99% | 95-102% | 86-95% | 93-101% |
| heparin plasma(n=5) | 84-101% | 92-101% | 78-96% | 87-104% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）50μL，
加入50μL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板5次；
4. 加TMB底物90μL，37°C孵育10-20分鐘；
5. 加終止液50μL，立即450nm讀數(shù)。
實驗原理
將抗原包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的免疫球蛋白G(IgG)與連接于固相載體上的抗原結(jié)合，然后加入HRP標(biāo)記的二抗，徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的免疫球蛋白G(IgG)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Animal and Veterinary Advances | Tryptophan Stimulates Immune Response in Broiler Chickens Challenged with Infectious Bursal Disease Vaccine [Medwell: 2010610616] |
| Journal of Animal and Veterinary Advances | Immunostimulatory Effects of Arginine in Broiler Chickens Challenged with Vaccine Strain of Infectious Bursal Disease Virus [Medwell: 2010594600] |
| international Journal of food and nutritional sciences | EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHI [Ijfans: Source] |
| Poultry Science | Effects of Clostridium butyricum on growth performance, immune function, and cecal microflora in broiler chickens challenged with Escherichia coli K88 [Pubmed: 24570422 ] |
| Pharmaceutical Development and Technology | Foot and Mouth Disease virus-loaded fungal chitosan nanoparticles for intranasal administration: impact of formulation on physicochemical and immunological characteristics [Pubmed: 23590209] |
| BioMed Research International | The Effect of CXC Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling [Hindawi:Source] |
| Journal of Proteomics | Proteomics on porcine haptoglobin and IgG/IgA show protein species distribution and glycosylation pattern to remain similar in PCV2-SD infection [Pubmed:24576640] |
| Int J Occup Environ Health. | Liver functions in silica-exposed workers in Egypt: possible role of matrix remodeling and immunological factors [Pubmed:24999850] |
| Food & Function | Dietary squid ink polysaccharide could enhance SIgA secretion in chemotherapeutic mice [Pubmed:25308407] |
| Oncology Reports | Beneficial effect of T follicular helper cells on antibody class switching of B cells in prostate cancer [Pubmed:25529861] |
| Biomed Res Int | The Effect of C-X-C Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling [PubMed: 26161394] |
| Protein Pept Lett | Stabilization of Human Immunoglobulin G Encapsulated within Biodegradable Poly (Cyclohexane-1, 4-diyl Acetone Dimethylene Ketal) (PCADK)/ Poly (Lactic-co-Glycolic Acid) (PLGA) Blend Microspheres [PubMed: 26268563] |
| Electrophoresis | Growth promotion in pigs by oxytetracycline coincides with down reCavia (Guinea pig )lation of serum inflammatory parameters and of hibernation‐associated protein HP‐27 [Pubmed:26914286] |
| Neuropeptides | Neuropeptide Y stimulates osteoblastic differentiation and VEGF expression of bone marrow mesenchymal stem cells related to canonical Wnt signaling activating in vitro. [Pubmed:26707636] |
| Archives of Biological Sciences | Effect of oral administration of Lactobacillus paracasei L9 on mouse systemic immunity and the immune response in the intestine [pid:46641600021Z] |
| Asian J Pharm Clin Res | INFLUENCE OF ZINCOVIT DROP (NUTRITIONAL FOOD SUPPLEMENT) ON IMMUNE SYSTEM IN NORMAL AND CYCLOPHOSPHAMIDE INTOXICATED WISTAR RATS [journals:3790] |
| journal of biotechnology | Expression of α-1,6-fucosyltransferase (FUT8) in rice grain and immunogenicity evaluation of plant-specific glycans. [pubmed:28013072] |
| Sci Rep. | Long-term use of ceftriaxone sodium induced changes in gut microbiota and immune system. [pubmed:28220870] |
| Toxicology Research | Effect of dietary protein or calcium supplement on the expression of collagen I and dentine phosphoprotein of rats with dental fluorosis [C6TX00288A] |
| ntervirology | Exposure of Piglets to Enteroviruses Investigated by an Immunoassay Based on the EV-G1 VP4 Peptide [pubmed:27694750] |
| Biomedicine & Pharmacotherapy | Icariin promotes osteogenic differentiation of rat bone marrow stromal cells by activating the ERα-Wnt/β-catenin signaling pathway. [pubmed:27764755] |
| Food and Agricultural Immunology | Effects of fermented cottonseed meal on growth performance, serum biochemical parameters, immune functions, antioxidative abilities, and cecal microflora in broilers [10.108:09540105.2017.1311308 ] |
| Biomedicine & Pharmacotherapy | In vitro and in vivo immunomodulatory potential of Pedicularis longiflora and Allium carolinianum in alloxan-induced diabetes in rats [pubmed:29091887] |
| Journal of Biochemical and Molecular Toxicology | Fluoride or/and aluminum induced toxicity in guinea pig teeth with the low expression of dentine phosphoprotein [10.1002/jbt.21912] |
| International Journal of Science and Research | Impact of Silver Nanoparticles on Immune Responses as Vaccine against Activated Staphylococcus aureus Bacteria [ISSN: 2319-7064] |
免疫球蛋白G(IgG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
編號CEA544Gu
物種Cavia (Guinea pig ，豚鼠) 相同的名稱，不同的物種。
實驗方法競爭抑制
反應(yīng)時長2h
檢測范圍1.23-100ug/mL
靈敏度最小可檢測劑量小于等于0.48ug/mL.
樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
下載英文說明書 中文說明書
規(guī)格48T96T 96T*5 96T*10 96T*100
價格￥ 1840
產(chǎn)品包裝圖模擬
特異性
本試劑盒用于檢測免疫球蛋白G(IgG)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的免疫球蛋白G(IgG)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 86-101 | 95 |
| EDTA plasma(n=5) | 90-102 | 97 |
| heparin plasma(n=5) | 84-91 | 87 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的免疫球蛋白G(IgG)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中免疫球蛋白G(IgG)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-96% | 80-98% | 86-105% | 89-101% |
| EDTA plasma(n=5) | 86-99% | 95-102% | 86-95% | 93-101% |
| heparin plasma(n=5) | 84-101% | 92-101% | 78-96% | 87-104% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）50μL，
加入50μL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板5次；
4. 加TMB底物90μL，37°C孵育10-20分鐘；
5. 加終止液50μL，立即450nm讀數(shù)。
實驗原理
將抗原包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的免疫球蛋白G(IgG)與連接于固相載體上的抗原結(jié)合，然后加入HRP標(biāo)記的二抗，徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的免疫球蛋白G(IgG)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Animal and Veterinary Advances | Tryptophan Stimulates Immune Response in Broiler Chickens Challenged with Infectious Bursal Disease Vaccine [Medwell: 2010610616] |
| Journal of Animal and Veterinary Advances | Immunostimulatory Effects of Arginine in Broiler Chickens Challenged with Vaccine Strain of Infectious Bursal Disease Virus [Medwell: 2010594600] |
| international Journal of food and nutritional sciences | EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHI [Ijfans: Source] |
| Poultry Science | Effects of Clostridium butyricum on growth performance, immune function, and cecal microflora in broiler chickens challenged with Escherichia coli K88 [Pubmed: 24570422 ] |
| Pharmaceutical Development and Technology | Foot and Mouth Disease virus-loaded fungal chitosan nanoparticles for intranasal administration: impact of formulation on physicochemical and immunological characteristics [Pubmed: 23590209] |
| BioMed Research International | The Effect of CXC Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling [Hindawi:Source] |
| Journal of Proteomics | Proteomics on porcine haptoglobin and IgG/IgA show protein species distribution and glycosylation pattern to remain similar in PCV2-SD infection [Pubmed:24576640] |
| Int J Occup Environ Health. | Liver functions in silica-exposed workers in Egypt: possible role of matrix remodeling and immunological factors [Pubmed:24999850] |
| Food & Function | Dietary squid ink polysaccharide could enhance SIgA secretion in chemotherapeutic mice [Pubmed:25308407] |
| Oncology Reports | Beneficial effect of T follicular helper cells on antibody class switching of B cells in prostate cancer [Pubmed:25529861] |
| Biomed Res Int | The Effect of C-X-C Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling [PubMed: 26161394] |
| Protein Pept Lett | Stabilization of Human Immunoglobulin G Encapsulated within Biodegradable Poly (Cyclohexane-1, 4-diyl Acetone Dimethylene Ketal) (PCADK)/ Poly (Lactic-co-Glycolic Acid) (PLGA) Blend Microspheres [PubMed: 26268563] |
| Electrophoresis | Growth promotion in pigs by oxytetracycline coincides with down reCavia (Guinea pig )lation of serum inflammatory parameters and of hibernation‐associated protein HP‐27 [Pubmed:26914286] |
| Neuropeptides | Neuropeptide Y stimulates osteoblastic differentiation and VEGF expression of bone marrow mesenchymal stem cells related to canonical Wnt signaling activating in vitro. [Pubmed:26707636] |
| Archives of Biological Sciences | Effect of oral administration of Lactobacillus paracasei L9 on mouse systemic immunity and the immune response in the intestine [pid:46641600021Z] |
| Asian J Pharm Clin Res | INFLUENCE OF ZINCOVIT DROP (NUTRITIONAL FOOD SUPPLEMENT) ON IMMUNE SYSTEM IN NORMAL AND CYCLOPHOSPHAMIDE INTOXICATED WISTAR RATS [journals:3790] |
| journal of biotechnology | Expression of α-1,6-fucosyltransferase (FUT8) in rice grain and immunogenicity evaluation of plant-specific glycans. [pubmed:28013072] |
| Sci Rep. | Long-term use of ceftriaxone sodium induced changes in gut microbiota and immune system. [pubmed:28220870] |
| Toxicology Research | Effect of dietary protein or calcium supplement on the expression of collagen I and dentine phosphoprotein of rats with dental fluorosis [C6TX00288A] |
| ntervirology | Exposure of Piglets to Enteroviruses Investigated by an Immunoassay Based on the EV-G1 VP4 Peptide [pubmed:27694750] |
| Biomedicine & Pharmacotherapy | Icariin promotes osteogenic differentiation of rat bone marrow stromal cells by activating the ERα-Wnt/β-catenin signaling pathway. [pubmed:27764755] |
| Food and Agricultural Immunology | Effects of fermented cottonseed meal on growth performance, serum biochemical parameters, immune functions, antioxidative abilities, and cecal microflora in broilers [10.108:09540105.2017.1311308 ] |
| Biomedicine & Pharmacotherapy | In vitro and in vivo immunomodulatory potential of Pedicularis longiflora and Allium carolinianum in alloxan-induced diabetes in rats [pubmed:29091887] |
| Journal of Biochemical and Molecular Toxicology | Fluoride or/and aluminum induced toxicity in guinea pig teeth with the low expression of dentine phosphoprotein [10.1002/jbt.21912] |
| International Journal of Science and Research | Impact of Silver Nanoparticles on Immune Responses as Vaccine against Activated Staphylococcus aureus Bacteria [ISSN: 2319-7064] |
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